SPECTROPHOTOMETRIC MEASUREMENT OF TRANSAMINATION REACTIONS
نویسندگان
چکیده
منابع مشابه
Spectrophotometric Measurement of Transamination
In a previous publication (1) from this laboratory, it was demonstrated that the transamination of amino acids to a-ketoglutaric acid is a reaction of wide scope. Although the data reported in the earlier paper permitted the computation of Q transaminase values, these values were of limited quantitative significance since no attempt was made to study the kinetics of these reaction systems, the ...
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Recent research has resulted in considerable modification of the earlier concept that enzymatic transamination was limited to reactions between alanine, aspartate, glutamate, and their or-keto analogues. Reactions leading to the reversible amination of the cY-keto analogues of many of the natural amino acids have been described (l-5), and there is also evidence that aldehyde groups may particip...
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A wide variety of mammalian cells in culture have been shown to require thirteen amino acids for survival and growth (l-4). The nonessential amino acids, not supplied in the medium, are synthesized by the cells from other components of the medium. Glucose’ and glutamine (5) have been shown to serve as sources of carbon. Since transamination is an important reaction in the interconversion and bi...
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The development of chemical transaminations as a new type of dynamic covalent reaction is described. The key 1,3-proton shift is under complete catalytic control and can be conducted orthogonally to, or simultaneous with, transimination in the presence of an amine to rapidly yield two-dimensional dynamic systems with a high degree of complexity evolution. The transamination-transimination syste...
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A simple, reliable and rapid method for the spectrophotometric determina tion of carboxyhemoglobin in fresh blood is described. A blood hemolysate is prepared by diluting a blood sample with 0.4 percent NH4 OH. Addition of sodium dithionite deoxygenates hemoglobin (and reduces methemoglobin if present) without affecting carboxyhemoglobin. Comparison of the 541/555 nm absorbance ratio for the h...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1951
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52426-7